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How to Find All Three Plasmium Samples in Hollow Knight: Silksong

Accept Zylotol’s Alchemist’s Assistant wish, charge the Needle Phial to harvest three Wormways buds, then return to the lab for the Plasmium Phial.
WorldHollow Knight: Silksong Length2 min Posted Quest giverVGSources Team
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To finish Alchemist Zylotol’s Alchemist’s Assistant Gather Wish, collect three Plasmium samples from buds in the Wormways and bring them back to his lab. Equip the temporary Needle Phial at the lab bench and fully charge it before striking each bud; a regular Needle hit will not harvest a sample. The first bud is below the lab. Directions to the second and third differ between guides, so the route below identifies which guide it follows.

Start Alchemist’s Assistant and prepare the Needle Phial

Find Alchemist Zylotol’s lab in the upper-left part of the Wormways and accept his Gather Wish. Rest at the bench in the lab, then equip the temporary Needle Phial in a tool slot. Hold the Tool input to charge it, then release the attack into a Plasmium bud. The Hollow Knight Wiki describes extraction as a fully charged stab; an ordinary Needle strike is not enough. Hollow Knight Wiki: Plasmium and Hollow Knight Wiki: Alchemist’s Assistant

Bud 1: below Zylotol’s lab

  1. Leave the lab to the right and drop to the level below.
  2. Turn left and use Wall Climb to cross the spike pit to a platform.
  3. Continue left and drop down. Look for a blue glow in an alcove on the right.
  4. Approach the bud, fully charge the Needle Phial, and release the attack to collect the sample.

This first-bud route is described by PCGamesN’s Plasmium guide.

Buds 2 and 3: guides disagree on the route

The detailed directions below follow the sequential route in Silksong Wiki. PCGamesN describes the second and third locations differently: it puts bud two in a dark western section behind a false wall and bud three in an eastern mossy cave. Those descriptions do not align cleanly with Silksong Wiki’s sequence and landmarks, so do not treat the two guides as a single confirmed route. The discrepancy is unresolved in the available guides.

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#1 Best Overall
Hollow Knight: Silksong Standard - Nintendo Switch [Digital Code]
  • Discover the fallen insect kingdom of Pharloom! Explore mossy grottos, gilded cities and misted moors as you ascend to the shining citadel at the top of the world.
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Bud 2: follow Silksong Wiki’s route through the moss-covered tunnel

From the first bud, go left and descend through the platforms, navigating the drops and spike hazards. Continue until you reach a moss-covered tunnel; Silksong Wiki places the second bud at the tunnel’s end. Charge the Needle Phial before striking it. See Silksong Wiki: Plasmium.

Bud 3: return toward the first falling sandworm

From bud two, climb back up toward the first falling sandworm. Cross left over a spike gap, then drop into an alcove and break the stone wall to reach the third bud. Fully charge the Needle Phial and release the attack into the bud to take the final sample. This is the third-bud route given by Silksong Wiki; PCGamesN’s location description differs.

Rank #2
Hollow Knight: Silksong | Standard Edition | Xbox Digital
  • Hollow Knight: Silksong is the epic sequel to Hollow Knight, the award winning action-adventure.
  • Journey to all-new lands, discover new powers, battle vast hordes of bugs and beasts and uncover secrets tied to your nature and your past.
Independent reader supportYour contribution helps us test, update, and keep practical guides available for everyone.Support on Ko-Fi

Return to Zylotol for the Plasmium Phial

With all three samples collected, return to the Wormways lab and speak with Zylotol. Completing the wish awards the Plasmium Phial. Hollow Knight Wiki: Alchemist’s Assistant

Quick Recap

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Classic side-scrolling action, with all the modern trimmings.
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  • Classic side-scrolling action, with all the modern trimmings.
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  • Forge your own path! The world of Hallownest is expansive and open. Choose which paths you take, which enemies you face and find your own way forward.
  • Evolve with powerful new skills and abilities! Gain spells, strength and speed. Leap to new heights on ethereal wings. Dash forward in a blazing flash. Blast foes with fiery Soul!.Equip Charms! Ancient relics that offer bizarre new powers and abilities. Choose your favourites and make your journey unique!.An enormous cast of cute and creepy characters all brought to life with traditional 2D frame-by-frame animation..

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