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Where to Find All 20 Glyphs in Assassin’s Creed II

A district-by-district guide to all 20 glyph locations in Assassin’s Creed II, plus how to activate their puzzles and what completing them reveals.
WorldAssassin's Creed II Length2 min Posted Quest giverVGSources Team
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Where do you find every glyph in Assassin’s Creed II? There are 20, spread across Florence, Monteriggioni, Tuscany, Romagna and Venice. You can collect them in any order; the numbered list below follows the order used by the GameFAQs location guide, not a required route. Spoilers: finding and solving all 20 reveals the complete video left by Subject 16.

To activate a glyph puzzle, stand in front of the glyph and use Eagle Vision. Finding a glyph and solving its puzzle are separate steps: the puzzles unlock in a fixed sequence, even if you collect the glyphs in a different order. The guide describes four recurring puzzle types: selecting a detail in an image, identifying five pictures that share a theme, rotating rings to complete an image, and using a cipher key and code wheel to derive a three-digit password.

For a map clue, the guide says a building image in the game database is marked with a red diamond containing an eye when it has a glyph.

Florence — San Marco District

  1. La Rosa Colta: Check the southern rooftop.
  2. Ospedale degli Innocenti: Check the eastern rooftop.
  3. Santa Croce: On the north side, on the lower roof section to the left when facing the front.

Florence — Santa Maria Novella District

  1. San Lorenzo church: At the church’s northern edge, between the dome and a bell tower.
  2. Mercato Vecchio: Go to the roof’s northeast corner and look down.

Monteriggioni

  1. Villa Auditore: On the front face, in the top-left corner.

Tuscany — Countryside

  1. Antico Teatro Romano: Inside a covered, tunnel-like section on the east side.
  2. Monte Oliveto Maggiore: On the east face of the tower.

Tuscany — San Gimignano

  1. Santa Maria Assunta: Face the front and go through the archway to the left of the main entrance.
  2. Torre del Diavolo: At the top.
  3. Torri dei Salvucci: Between the two towers.

Romagna

  1. San Mercuriale: Above the courtyard on the west side.
  2. Lighthouse tower: At the far northeast of the map, on the outside below the viewpoint. The guide places it north of the building whose location note you obtain.

Venice — San Polo District

  1. San Giacomo di Rialto: On top, between the dome and bell tower.
  2. Ponte di Rialto: Beneath the east side of the bridge. Swim underneath to see the glyph.
  3. Gilda dei Ladri di Venezia: On the rooftop just north of the entrance to the main office.

Venice — San Marco District

  1. Campanile di San Marco: In an open section on the tower’s top floor, near the top.
  2. Torre dell’Orologio: On the roof directly above the clock.

Venice — Castello District

  1. Scuola Grande di San Marco: On top.
  2. San Pietro di Castello: On the east side.

The landmark names and location cues follow the GameFAQs guide; its numbering is simply the author’s discovery order. Its listed locations are practical community-guide directions, not developer statements.

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